The Neurobiologist's Guide to Structural Biology: A Primer on Why Macromolecular Structure Matters and How to Evaluate Structural Data

نویسنده

  • Daniel L. Minor
چکیده

Structural biology now plays a prominent role in addressing questions central to understanding how excitable cells function. Although interest in the insights gained from the definition and dissection of macromolecular anatomy is high, many neurobiologists remain unfamiliar with the methods employed. This primer aims to help neurobiologists understand approaches for probing macromolecular structure and where the limits and challenges remain. Using examples of macromolecules with neurobiological importance, the review covers X-ray crystallography, electron microscopy (EM), small-angle X-ray scattering (SAXS), and nuclear magnetic resonance (NMR) and biophysical methods with which these approaches are often paired: isothermal titration calorimetry (ITC), equilibrium analytical ultracentifugation, and molecular dynamics (MD).

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Introducing a Lightweight Structural Model via Simulation of Vernacular “Pa Tu Pa” Arch

The knowledge of Iranian vernacular structures is based on geometry, and there is a possibility of recreating such structural patterns aimed at producing movable structures. The purpose of this research was to utilize the patterns of vernacular structures to provide a lightweight structural model. The questions raised included how to create various forms based on the structural history of any r...

متن کامل

سازه، فرم و معماری

The architectural form is affected by numerous factors including functional, economic, socio-cultural, climatic as well as structural requirements and stability. Among these factors, structure receives considerable attention as it has a critical role in generating form and its stability. Stability has always been an essential property of architectural forms, thus it is crucial to study structur...

متن کامل

Widdowson and Classroom Discourse

  Drawing on recent developments in linguistic description and applied linguistics, it can be concluded that learning a language necessitates getting to know something and being able to do something with that knowledge: competence, and performance. Structural approach to language description attaches importance to the former; communicative approach to the latter. Appropriate classroom discours...

متن کامل

An efficient CAD tool for High-Level Synthesis of VLSI digital transformers

Digital transformers are considered as one of the digital circuits being widely used in signal and data processing systems, audio and video processing, medical signal processing as well as telecommunication systems. Transforms such as Discrete Cosine Transform (DCT), Discrete Wavelet Transform (DWT) and Fast Fourier Transform (FFT) are among the ones being commonly used in this area. As an illu...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

عنوان ژورنال:
  • Neuron

دوره 54  شماره 

صفحات  -

تاریخ انتشار 2007